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Image Search Results
Journal: Oncology Letters
Article Title: Anticancer effect of arsenite on cell migration, cell cycle and apoptosis in human pancreatic cancer cells
doi: 10.3892/ol.2016.4564
Figure Lengend Snippet: Effects of PDGF-BB on the migration of AsPC-1 and BxPC-3 pancreatic cancer cells, compared with normal PE cells. (A) AsPC-1, (B) BxPC-3 and (C) PE cells (5×104 cells/well) were treated with the indicated doses of arsenite for 24 h, prior to be seeded in the upper Boyden chamber. Following incubation for 16 h, cells were exposed to 30 ng/ml PDGF-BB for 36 h at 37°C. Cells were then fixed, stained and visualized under a microscope. The average number of migrated cells from five randomly selected fields on the lower surface of the membrane was counted. Data were obtained from ≥3 independent experiments. *P<0.05 vs. controls. Right panels show representative images of the migrated cells stained with clonogenic reagent. PDGF, platelet-derived growth factor; PE, pancreatic epithelial.
Article Snippet:
Techniques: Migration, Incubation, Staining, Microscopy, Membrane, Derivative Assay
Journal: Oncology Letters
Article Title: Anticancer effect of arsenite on cell migration, cell cycle and apoptosis in human pancreatic cancer cells
doi: 10.3892/ol.2016.4564
Figure Lengend Snippet: Effects of arsenite on PARP cleavage in AsPC-1, BxPC-3 and PE cells. (A) AsPC-1, (B) BxPC-3 and (C) PE cells were exposed to arsenite (30 µM) for the indicated time periods. Protein extracts were then harvested and examined by western blotting using anti-PARP and anti-glyceraldehyde-3-phosphate dehydrogenase antibodies. Background-subtracted signal intensity of each protein band was normalized to GAPDH. Data are presented as the mean ± standard deviation of triplicate assay. *P<0.05. PARP, poly(adenosine diphosphate-ribose) polymerase; PE, pancreatic epithelial; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
Article Snippet:
Techniques: Western Blot, Standard Deviation
Journal: Oncology Letters
Article Title: Anticancer effect of arsenite on cell migration, cell cycle and apoptosis in human pancreatic cancer cells
doi: 10.3892/ol.2016.4564
Figure Lengend Snippet: Effects of arsenite on the phosphorylation of p44/p42 MAPK and Akt in AsPC-1, BxPC-3 and PE cells. (A) AsPC-1, (B) BxPC-3 and (C) PE cells were exposed to arsenite (30 µM) and incubated with 30 ng/ml platelet-derived growth factor-BB for the indicated time periods. Protein extracts were then harvested and examined by western blotting using specific antibodies against phospho-p44/p42 MAPK, p44/p42 MAPK, phospho-Akt and Akt. Background-subtracted signal intensity of each protein band was normalized to Akt. Data are presented as the mean ± standard deviation of triplicate assay. *P<0.05 vs. cells without arsenite exposure. PE, pancreatic epithelial; PDGF, platelet-derived growth factor; phospho, phosphorylated; MAPK, mitogen-activated protein kinase; M, marker.
Article Snippet:
Techniques: Phospho-proteomics, Incubation, Derivative Assay, Western Blot, Standard Deviation, Marker
Journal: PPAR Research
Article Title: Targeting the EZH2-PPAR Axis Is a Potential Therapeutic Pathway for Pancreatic Cancer
doi: 10.1155/2021/5589342
Figure Lengend Snippet: The protein expression levels of EZH2, H3K27ac, and H3K27me3 in cells of PANC-1 and AsPC-1. (a) Representative bands of western blot of EZH2, H3K27ac, H3K27me3, and histone H3 in cells of H6C7, PANC-1, and AsPC-1. (b) The relative band intensity of EZH2 in cells of H6C7, PANC-1, and AsPC-1. (c) The relative band intensity of H3K27ac in cells of H6C7, PANC-1, and AsPC-1. (d) The relative band intensity of H3K27me3 in cells of H6C7, PANC-1, and AsPC-1. (e) The relative mRNA level of EZH2 in cells of H6C7, PANC-1, and AsPC-1. The values were expressed as the means ± S.E.M. ( n = 6 for each group). ∗ P < 0.05 vs. the H6C7 group.
Article Snippet: Human normal pancreatic ductal epithelial cell line of
Techniques: Expressing, Western Blot